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Anabolic steroid injection into vein
This system involved the administration of anabolic steroids on rats, either orally or by injection (depending on the anabolic steroid being assessed)at either the early or late stages. In vivo experiments used acute experiments in which animals were administered either a 0.25% or 2% saline solution, while in vivo experiments used repeated injections over time (as in a test, e.g. running wheel test) that were delivered in 4-hour cycles until a specific time in the cycle when the injection was delivered.
The rat used [ 18 F]FSH was obtained from the Charles River Life Sciences Laboratory, Inc., Wilmington, MA. The control animals were housed in groups housed separately and used to simulate a sexually reproducing animal, where each animal was individually housed during the experiment, anabolic steroid injection thigh. The control rats were used to approximate the control condition that rats would be in during normal reproductive times, best place to inject steroids for maximum results. A control group of male Sprague-Dawley rats, not involved in this study, was also housed in groups. The other animals that acted as controls were used for an additional set of experiments. For example, rats that were used as controls for a given rat in one set of experiments were used to mimic a new situation, such as the test for a new drug on a given date, and were also used in an additional set of experiments that involved a test in a different location on the same date, anabolic steroid injection into vein.
To evaluate the effects of anabolic steroids on the hypothalamic-pituitary-gonadal axis, androgens were evaluated with serum hormones (androstenedione, LH, FSH), gonadotropin-releasing hormone (GnRH), and testosterone, by using intraperitoneal (iP) injections of the respective compounds. The animals were given daily injections twice a day starting on the same day that an animal became pregnant at 1 week of age (approximately 30–45 days after the last injection), injection steroid anabolic into vein. In some experiments a group of animals was used to mimic the conditions of a sexually reproducing rat, where each animal was individually housed during the experiment and had normal reproductive cycles. If the animals were used for further studies and/or an endocrinology laboratory was available, the individual animals were used to simulate the conditions of a sexually reproducing human. The rats' reproductive cycles were recorded by use of periodic weighing or ultrasonography of the testes to measure the percentage of testes that were swollen during testing, anabolic steroid is testosterone. A standard curve using the Wilcoxon's test was also used to determine the average increase (in percentage) of total body weight over time for each individual rat.
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